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CellTracker is an image processing software to perform automated, semi-automated, and manual cell migration detection.

The main goals of the software are:

• automated image quality enhancement using vignetting and alignment correction;
• detection and tracking of cells;
• editing cell paths and statistical analysis of the cell motion.
  

The major challenge what we addressed is to perform the above goals on phase-contrast and DIC images. Nevertheless, CellTracker works on fluorescent images as well.

The program is written in MatLab with a graphical user interface, it requires MatLab 2012, Image Processing Toolbox 8.1 and for some optional functions Curve Fitting Toolbox 3.3 or later versions. The output statistics are stored in human readable table file (Excel, .csv). Furthermore, it is possible to create movies and visualize migration over time in 3D graphs (x, y, time).

How to cite us...  
Piccinini, F., Kiss, A., Horvath, P. (2015), CellTracker (not only) for dummies. Bioinformatics, doi: 10.1093/bioinformatics/btv686
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Tracking cells in phase-contrast and DIC image is now easy!

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Hungarian Academia of Sciences
Biological Research Centre, 
Szeged, Hungary

Institute for Molecular Medicine Finland 
University of Helsinki, 
Helsinki, Finland